| 1. | Bioconversion of conjugated linoleic acid by resting cells of lactobacillus plantarum zs2058 in potassium phosphate buffer system 2058在磷酸盐缓冲液体系中生物转化共轭亚油酸 |
| 2. | The supernatants were dialyzed against 50 mm phosphate buffer ( pb ) ( ph 6 . 5 ) , and passed through a blue - sepharose 6b column ( 3x30 cm ) Iptg诱导表达目的蛋白,上清液经sds一page电泳后看到约32kd的目的条带。 |
| 3. | The electrochemical and spectroscopic behaviors of mp - 11 in the in the aqueous solution and simulated physiological solution , i . e . the solution of 20 % methanol and phosphate buffer solution with 20 % methanol are compared 比较了mp ? 11在水溶液和模拟生理条件(即20甲醇和5mmol / l磷酸缓冲溶液)中的电化学和光谱行为。 |
| 4. | Degradation in vitro of cs rod was studied . results of weight loss and molecular weight change of cs rod degrading in phosphate buffered solution for 16w indicated that cs degraded very slowly 对壳聚糖棒材体外降解进行了初步的研究,发现棒材在磷酸盐降解液中16w后失重率和分子量降低不明显,表明壳聚糖降解非常缓慢。 |
| 5. | 4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100 , g50 ) . at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod 因而不管从第一次的纯化来看,还是从文献上报道的来看,本实验的串联柱层析是较为合理有效的一种生产方法,值得推广。 |
| 6. | The films were soaked in sbf simulated body fluid and cpbs phosphate buffer solution , the results showed that the fha films were less soluble than ha films . with the increase of fluorine content , the fha sediment layer in sbf solution becomes more even Fha薄膜sbf浸泡实验结果表明:随着薄膜中氟含量的增加,薄膜表面上羟浙江大学硕士学往论文基磷灰石沉积层越来越均匀,沉积方式由岛状向层状转变,说明生物活性随之增加。 |
| 7. | Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus 病毒感染后的8h , 12h , 16h , 20h提取中肠组织,按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序,制成连续切片,在倒置显微镜下逐一进行观察计数。 |
| 8. | The results proved the feasibility of this enrichment technique . the enriched nitrifying bacteria can directly store in a lucifugal condition for a long time , the nitrifiers decay at a rate of 0 . 0138d - 1 . but the decay rate of the nitrifiers stored in phosphate buffer ( ph 7 . 2 ) is 0 . 023d - 1 硝化细菌富集培养后直接避光保存是一种较佳的保存方式,经3个月的保存其硝化细菌存活率为26 . 7 ,其比衰减率为0 . 0138d ~ ( - 1 )硝化细菌。 |
| 9. | Without ammonium sulphate fractionation and dialysis , the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l , ph7 . 8 ) , and the enzyme fraction was not absorbed on the column but impurities were absorbed 粗酶液无需硫酸铵沉淀及透析,即可引入磷酸缓冲液( 50mmol l , ph7 . 8 )预平衡的deae - sepharosecl6b柱,上柱后用平衡缓冲液洗至基线稳定。 afpga不被吸附而直接流出。 |
| 10. | Scanning electron microscopy : after fixed in 2 . 5 % glutaraldehyde made up in 0 . 1 m phosphate buffer ( ph 7 . 2 ) , the tissue of testis was post - fixed in 1 . 0 % oso4 , dehydrated in a progressive ethanol solution , dried and sputter - coated with gold , then observed with kyky - 1000b microscopy and photographed 扫描电镜样品以2 . 5戊二醛( ph7 . 2 , 0 . 1m lpbs缓冲液配制)和1锇酸双固定,酒精系列脱水,干燥,真空离子溅射仪喷金, kyky - 1000b型扫描电镜观察并拍照。 |